pbabe hygro htert plasmid (Addgene inc)
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pbabe hygro htert plasmid
Pbabe Hygro Htert Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbabe+hygro+htert+plasmid/pBABE-hygro-hTERT+(Plasmid+%231773)/pmc12961426-53-23-25
Average 93 stars, based on 103 article reviews
Pbabe Hygro Htert Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbabe+hygro+htert+plasmid/pBABE-hygro-hTERT+(Plasmid+%231773)/pmc12961426-53-23-25
Average 93 stars, based on 103 article reviews
pbabe hygro htert plasmid - by Bioz Stars,
2026-10
93/100 stars
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Plasmid Preparation:Article Title: Adipose-Derived Mesenchymal Stem Cell (MSC) Immortalization by Modulation of hTERT and TP53 Expression Levels. Article Snippet: Stained cells were analyzed on a BD FACS Aria III (BD Bioscience, USA) flow cytometer. .. For retrovirus assembly, the Article Title: MnTE-2-PyP protects fibroblast mitochondria from hyperglycemia and radiation exposure. Article Snippet: .. P3158 are normal human prostate fibroblasts that were immortalized by Article Title: Control of retrotransposon-driven activation of the interferon response by the double-stranded RNA binding protein DGCR8 Article Snippet: Both were cultured in Gibco MEM, 15% heat-inactivated foetal bovine serum, 100 U/mL penicillin-streptomycin and 0.1 mM Non-Essential Amino Acids (Gibco). .. To achieve cellular immortalization, retroviral particles encoding the human telomerase reverse transcriptase (hTERT) were generated by transfecting Phoenix helper-free packaging cells with the Article Title: Manganese porphyrin, MnTE-2-PyP, treatment protects the prostate from radiation-induced fibrosis (RIF) by activating the NRF2 signaling pathway and enhancing SOD2 and sirtuin activity Article Snippet: .. Immortalization was performed by using Article Title: MnTE-2-PyP, a manganese porphyrin, reduces cytotoxicity caused by irradiation in a diabetic environment through the induction of endogenous antioxidant defenses Article Snippet: .. P3158 cells are cultured from primary prostate tissue collected from the prostate of a heathy male and immortalized using a Article Title: MnTE-2-PyP protects fibroblast mitochondria from hyperglycemia and radiation exposure Article Snippet: .. P3158 are normal human prostate fibroblasts that were immortalized by Article Title: Control of retrotransposon-driven activation of the interferon response by the double-stranded RNA binding protein DGCR8 Article Snippet: Both were cultured in Gibco MEM, 15% heat-inactivated FBS, 100 U/mL penicillin-streptomycin, and 0.1 mM Non-Essential Amino Acids (Gibco). .. To achieve cellular immortalisation, retroviral particles encoding the human telomerase reverse transcriptase (hTERT) were generated by transfecting Phoenix helper-free packaging cells with the Retroviral:Article Title: Control of retrotransposon-driven activation of the interferon response by the double-stranded RNA binding protein DGCR8 Article Snippet: Both were cultured in Gibco MEM, 15% heat-inactivated foetal bovine serum, 100 U/mL penicillin-streptomycin and 0.1 mM Non-Essential Amino Acids (Gibco). .. To achieve cellular immortalization, retroviral particles encoding the human telomerase reverse transcriptase (hTERT) were generated by transfecting Phoenix helper-free packaging cells with the Article Title: Control of retrotransposon-driven activation of the interferon response by the double-stranded RNA binding protein DGCR8 Article Snippet: Both were cultured in Gibco MEM, 15% heat-inactivated FBS, 100 U/mL penicillin-streptomycin, and 0.1 mM Non-Essential Amino Acids (Gibco). .. To achieve cellular immortalisation, retroviral particles encoding the human telomerase reverse transcriptase (hTERT) were generated by transfecting Phoenix helper-free packaging cells with the Reverse Transcription:Article Title: Control of retrotransposon-driven activation of the interferon response by the double-stranded RNA binding protein DGCR8 Article Snippet: Both were cultured in Gibco MEM, 15% heat-inactivated foetal bovine serum, 100 U/mL penicillin-streptomycin and 0.1 mM Non-Essential Amino Acids (Gibco). .. To achieve cellular immortalization, retroviral particles encoding the human telomerase reverse transcriptase (hTERT) were generated by transfecting Phoenix helper-free packaging cells with the Article Title: Control of retrotransposon-driven activation of the interferon response by the double-stranded RNA binding protein DGCR8 Article Snippet: Both were cultured in Gibco MEM, 15% heat-inactivated FBS, 100 U/mL penicillin-streptomycin, and 0.1 mM Non-Essential Amino Acids (Gibco). .. To achieve cellular immortalisation, retroviral particles encoding the human telomerase reverse transcriptase (hTERT) were generated by transfecting Phoenix helper-free packaging cells with the Generated:Article Title: Control of retrotransposon-driven activation of the interferon response by the double-stranded RNA binding protein DGCR8 Article Snippet: Both were cultured in Gibco MEM, 15% heat-inactivated foetal bovine serum, 100 U/mL penicillin-streptomycin and 0.1 mM Non-Essential Amino Acids (Gibco). .. To achieve cellular immortalization, retroviral particles encoding the human telomerase reverse transcriptase (hTERT) were generated by transfecting Phoenix helper-free packaging cells with the Article Title: Control of retrotransposon-driven activation of the interferon response by the double-stranded RNA binding protein DGCR8 Article Snippet: Both were cultured in Gibco MEM, 15% heat-inactivated FBS, 100 U/mL penicillin-streptomycin, and 0.1 mM Non-Essential Amino Acids (Gibco). .. To achieve cellular immortalisation, retroviral particles encoding the human telomerase reverse transcriptase (hTERT) were generated by transfecting Phoenix helper-free packaging cells with the Transfection:Article Title: Control of retrotransposon-driven activation of the interferon response by the double-stranded RNA binding protein DGCR8 Article Snippet: Both were cultured in Gibco MEM, 15% heat-inactivated foetal bovine serum, 100 U/mL penicillin-streptomycin and 0.1 mM Non-Essential Amino Acids (Gibco). .. To achieve cellular immortalization, retroviral particles encoding the human telomerase reverse transcriptase (hTERT) were generated by transfecting Phoenix helper-free packaging cells with the Article Title: Control of retrotransposon-driven activation of the interferon response by the double-stranded RNA binding protein DGCR8 Article Snippet: Both were cultured in Gibco MEM, 15% heat-inactivated FBS, 100 U/mL penicillin-streptomycin, and 0.1 mM Non-Essential Amino Acids (Gibco). .. To achieve cellular immortalisation, retroviral particles encoding the human telomerase reverse transcriptase (hTERT) were generated by transfecting Phoenix helper-free packaging cells with the Cell Culture:Article Title: MnTE-2-PyP, a manganese porphyrin, reduces cytotoxicity caused by irradiation in a diabetic environment through the induction of endogenous antioxidant defenses Article Snippet: .. P3158 cells are cultured from primary prostate tissue collected from the prostate of a heathy male and immortalized using a |
![Biological activity evaluation of RHPS4 and Pyridostain. ( A , B ) Immunofluorescence evaluation of G4 structures in human cervical HeLa (A) and osteosarcoma U2OS (B) cancer cell lines. The cells were treated with the telomere-directed pentacyclic acridine 3,11-difluoro-6,8,13-trimethyl-8H-quino[4,3,2-kl]acridinium methosulfate (RHPS4) and the non-telomere-directed N,N′-bis (quinolinyl)pyridine-2,6-dicarboxamide (Pyridostatin, PDS) at the final concentration of 0.5 μM for 24 h and then processed for immunofluorescence with the specific antibody for G4 structures (anti-BG4). Left panel s : representative images acquired by confocal microscopy (magnification 63×). G4 structures are labeled in red and the nuclei are stained with DAPI (blue). 4× enlargements of selected regions (white squares) are shown. Scale bars indicate 20 μm. Right panels: Quantification of the number of G4 structures per cell. At least 30 cells for condition were counted. The histograms represent the mean values ± S.D. of three independent experiments. * P < .05, ** P < .01, and *** P < .001. ( C , D ) Cell viability assays performed in human foreskin-derived fibroblast (BJ), expressing the human telomerase reverse transcriptase hTERT and SV40 early region (BJ-EHLT) or only hTERT (BJ-hTERT) and treated with PDS and RHPS4 at the final concentrations of 0.1, 0.5, and 1 μM for 48 (C) and 96 (D) h. The results were expressed as the percentage of cell viability over the untreated cells. (E–G) BJ-EHLT cells were treated with the indicated compound for 24 h at a final concentration of 0.5 μM and, successively, processed for telomeric FISH combined with immunofluorescence experiments using the antibody against γH2AX, a marker of the DNA damage. ( E ) Representative images acquired by confocal microscopy (magnification 63×). Scale bars indicate 10 μm. ( F ) Percentage of γH2AX positive cells. ( G ) Quantitative analysis of the number of TIF per cell and of the percentage of TIF positive cells. Cells with at least four γ-H2AX/Telo colocalizations were scored as TIF positive. The histograms represent the mean values ± S.D. of three independent experiments. * P < .05, ** P < .01, *** P < .001, and **** P < .0001.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_0870/pmc12000870/pmc12000870__gkaf301fig7.jpg)